N.D. Pajo, K. l. Pua, A.Yoppo, E. R Ejiofo Accessions, polymorphic, dendrogram, clusters, tef, ISSR 132-138 9 (4) 2025 October, 2025 Kola (Cola spp.) is a valuable tree crop with significant nutritional, medicinal, and industrial importance. This study employed Inter-Simple Sequence Repeat (ISSR) markers to assess the genetic diversity of 20 Nigerian kola accessions. The analysis revealed a high degree of polymorphism, with primers generating 5 to 17 alleles each. The average Polymorphic Information Content (PIC) was 0.749, indicating high marker informativeness, while the gene diversity was 0.767. A UPGMA dendrogram clustered the accessions into five distinct groups, with a similarly coefficient of 0.72 confirming substantial genetic variation. These results demonstrate that ISSR markers are highly effective for the genetic characterization of kola, revealing phylogenetic relationships and identifying variability that can be harnessed for cultivar development and crop improvement.