Sudip Neupane, Radhika Regmi Diversity, rapeseed, SSR marker, polymorphism information content 171-179 4 (4) 2020 October, 2020 Rapeseed is an important oilseed crop in Nepal which covers significant position among cash crops in terms of area and production. We have used already published SSR markers to study the genetic diversity among the rapeseed germplasm accessions. Altogether 20 SSR primers were tested for 48 Nepalese rapeseed germplasms. Out of 48 SSR markers, 11 markers showed polymorphism. Polymorphism Information Content (PIC) value ranged from 0.40 to 0.84. Out of eleven polymorphic markers, NA10-C01a showed the highest level of polymorphism (PIC=8.4). Percent of polymorphic marker was 55% where alleles per polymorphic loci were 2.36 and the sum of total polymorphic alleles was 26. The cluster diagram categorized studied germplasm and cultivars in two major groups with maximum similarity of 36.05%. Hundred percent similarities were observed between genotypes collected from Ramechhap (Rato Tori) region with accession number NGCRO2790 and Sindhupalchok (Sano Tori). We also noted 100 % similarity between the two released varieties, namely Sumle Tori 3 and Bikas, whereas more than 90% similarity was observed between the three released varieties Unnati, Sumle Tori and Bikas. The Principle Component Analysis (PCA) also support the findings obtained by cluster analysis, where all the genotypes also characterized in two major groups. We conclude that greater diversity is present among the studied accessions and SSR markers are very handy and applicable tool for the diversity assessment of rapeseed germplasm. Therefore we can exploit the available rapeseed diversity of the country for the development of better rapeseed varieties to cope with biotic and abiotic stresses and climate change issues.